Coding

Part:BBa_K3617007:Design

Designed by: David Nørgaard Essenbæk   Group: iGEM20_UCopenhagen   (2020-10-21)


TMDWsc1-TEV_recseq-LexA-VP16


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 507
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We also had other ways of localizing the transcription factor to the plasma membrane e.g. with a C-terminal CAAX box. In the end this design fitted better to our cloning flow since it reuses some of the protein domains also used elsewhere in our project.


Source

S. cerevisiae, E. coli, herpes simplex virus.

References